Strain Data Sheet

RBRC10201

Strain Information

Image
BRC No.RBRC10201
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameCB17.Cg-Plxnb1<tm1Matl>
Former Common nameCB17-PlexinB1-KO
H-2 Haplotype
ES Cell lineE14TG2a [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Roland H. Friedel and deposited by Shinobu Inagaki, Osaka University Graduate School of Medicine, Division of Health Sciences. E14TG2a ES cells derived from 129P2/OlaHsd were used. This strain was backcrossed to C.B-17/lcr-+/+Jcl for 10 generations.
Strain descriptionPlxnb1 (plexin B1) knockout mice. C.B-17/lcr congenic strain.
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 102(37) 13188-13193 (2005). 16129827

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Plxnb1plexin B19Plxnb1<tm1Matl>targeted mutation 1, Marc Tessier-Lavigne

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
CD4Rat CD4 cDNA9CD4

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)9En2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)9

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)9

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene9

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
human Placental alkaline phosphatase cDNA9

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA9

Ordering Information

Donor DNAMouse En2 splice acceptor, Rat CD4 cDNA, E.coli Galactosidase/neomysin phosphotransferase fusion gene (β-geo), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Human Placental alkaline phosphatase cDNA, SV40 Poly A, mouse Plxnb1 genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA 102: 13188-13193 (2005).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. 　RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorDrs. Tatsuo Furuyama and Shinobu Inagaki (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data