Strain Information | |
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Image | |
BRC No. | RBRC10222 |
Type | CRISPR/Cas9 |
Species | Mus musculus |
Strain name | BALB/c-S100a9<em1Fjw> |
Former Common name | MRP14-KO BALB/cA mice |
H-2 Haplotype | |
ES Cell line | |
Background strain | BALB/cAJcl |
Appearance | |
Strain development | Developed by Wataru Fujii and Yasuyuki Goto, The University of Tokyo in 2017. Fertilized eggs derived from BALB/cAJcl was used. BALB/cAJcl genetic background. |
Strain description | S100a9 [S100 calcium binding protein A9 (calgranulin B)] mutant mice generated by the CRISPR/Cas9. 338 bp genomic fragment covering exon 2 was deleted. Deleted genomic sequence, (Chr 3: 90695035 to 90695372 (GRCm38.p4))TGGCCAGAGACTAGGTCAGGGAAGCTTGGTATCCCATTCTTTTATTTGTAACTTTTGTTTCCCCTATTTTCAAAGAAGAGCAAGAAGATGGCCAACAAAGCACCTTCTCAGATGGAGCGCAGCATAACCACCATCATCGACACCTTCCATCAATACTCTAGGAAGGAAGGACACCCTGACACCCTGAGCAAGAAGGAATTCAGACAAATGGTGGAAGCACAGTTGGCAACCTTTATGAAGGTGAGGCTTGGCTTGTGAGAGAAGAGGGTGCAATCTGCAGCAGTCTGAGAACAAATTCTAGCACCGGGGGCAGTAGGGCTCTGCCATTAGAGGCACTC |
Colony maintenance | |
References | Immunol. Lett., 194, 13-20 (2017). 29253495 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter S100a9S100 calcium binding protein A9 (calgranulin B)3S100a9<em1Fjw>endonuclease-mediated mutation 1, Wataru Fujii |
Ordering Information | |
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Donor DNA | |
Research application | |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunol. Lett., 194, 13-20 (2017). |
Depositor | Yasuyuki Goto (The University of Tokyo) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |