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AES0146  :  Dnmt1-/-Dnmt3a-/-Dnmt3b-/- ES (clone19)
Comment Mouse ES cell line lacking all of Dnmt1, Dnmt3a and Dnmt3b. Derived from 129Sv/Jae.
Animal mouse, 129Sv/Jae
Sex Male
Age embryo
Tissue embryonic stem cells
Exogene LacZ, Neomycin resistance gene, Hygromycin resistance gene, Puromycin resistance gene, IRES
Morphology ES-like
Anchoraged Yes
Medium GMEM+15%FBS+0.1mM NEAA+1mM Sodium Pyruvate+1000U/ml mouse LIF+0.1mM 2-Mercaptoethanol
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin+0.02% EDTA, 0.1% gelatin coated dish
Split ratio 1:4-10 split
Subculture frequency once/2-3days
Cloned No
Lifespan infinite
Mycoplasma -
Isozyme analysis LD, NP
memo Misprint on ampoules. Dnmt1-/-Dnmt3a-/-Dnmt3b-/- ES (clone19) -> mDnmtTKO-AT19
Originator Tsumura, Akiko & Okano, Masaki
Depositor Okano, Masaki
Restriction In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Genes Cells 2006;11:805-814) designated by the DEPOSITOR is required. f
Photo 2014-06-26
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