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CELL SEARCH SYSTEM

AES0146  :  Dnmt1-/-Dnmt3a-/-Dnmt3b-/- ES (clone19)
Comment Mouse ES cell line lacking all of Dnmt1, Dnmt3a and Dnmt3b. Derived from 129Sv/Jae.
Animal mouse, 129Sv/Jae
Sex Male
Age embryo
Tissue embryonic stem cells
Exogene LacZ, Neomycin resistance gene, Hygromycin resistance gene, Puromycin resistance gene, IRES
Morphology ES-like
Anchoraged Yes
Medium GMEM+15%FBS+0.1mM NEAA+1mM Sodium Pyruvate+1000U/ml mouse LIF+0.1mM 2-Mercaptoethanol
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin+0.02% EDTA, 0.1% gelatin coated dish
Split ratio 1:4-10 split
Subculture frequency once/2-3days
Cloned No
Lifespan infinite
Mycoplasma -
Isozyme analysis LD, NP
memo Misprint on ampoules. Dnmt1-/-Dnmt3a-/-Dnmt3b-/- ES (clone19) -> mDnmtTKO-AT19
Originator Tsumura, Akiko & Okano, Masaki
Depositor Okano, Masaki
Restriction In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Genes Cells 2006;11:805-814) designated by the DEPOSITOR is required. f
Photo 2014-06-26
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank@brc.riken.jp) regarding any kind of for-profit use.


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