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APS0001  :  iPS-MEF-Ng-20D-17
Comment Mouse induced pluripotent stem (iPS) cell line. Expressing GFP by Nanog promoter. Order Form ( C-0033, C-00034). Please see CiRA , Kyoto University if you are a researcher at a for–profit organization.
Animal mouse
Sex Male
Age embryo
Tissue embryonic fibroblast
Exogene pMXs, Oct3/4, Sox2, Klf4, c-Myc, Nanog-GFP-IRES-Puro^r
Morphology ES-like
Anchoraged Yes
Medium DMEM(high glucose, without Sodium Pyruvate)+15%FBS+0.1mM NEAA+0.1mM 2-Mercaptoethanol+1000U/ml mouse LIF
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin+0.02% EDTA, 0.1% gelatin coated dish
Split ratio 2x10^(6) cells/100mm dish
Subculture frequency SC : once/2-3days, MC : every day
Cloned Yes
Lifespan infinite
Mycoplasma -
Isozyme analysis Lot.1: LD, NP
memo feeder : MEF (X-rays:5000R or MMC) 1-2x10^(6) cells/100mm dish
Originator Yamanaka, Shinya
Restriction 1) The RECIPIENT should read ' the GFP Transfer License contracted between the RIKEN BRC and GE Healthcare Bio-Sciences.' 2) Attach TERMS and CONDITIONS to MTA (C-0034). GFP,f
Photo 2014-05-08
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.

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