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HES0001  :  KhES-1
Comment Human embryonic stem cell line.
Animal human
Sex Female
Age embryo
Tissue embryonic stem cells
Morphology ES-like
Anchoraged Yes
Medium DMEM/HamF12(2mM L-Glutamine)+20%KSR+0.1mM NEAA+0.1mM 2-Mercaptoethanol+5ng/ml human bFGF
Antibiotics Free
Growth temp 37oC
CO2 concentration 3%
Passage method 0.25% trypsin+0.1% collagenase type IV+20% KSR+1mM CaCl2+PBS(-), 0.1% gelatin coated dish
Split ratio 1:2-4 split
Subculture frequency subculture : once/3-4days. medium change : every day
Lifespan infinite
Mycoplasma -
Short Tandem Repeat OK
memo DAP213 : Medium+2M DMSO+1M Acetamide+3M Propyleneglycol, feeder : MEF (X-rays:5000R or MMC) 3-5x10^(5) cells/60mm dish
Originator Nakatsuji, Norio
Depositor Nakatsuji, Norio
Restriction Please read carefully the Japanese governmental guidelines on the utilization of human embryonic stem (ES) cells . b
Photo 2014-05-15
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.

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