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CELL SEARCH SYSTEM

HPS0063  :  201B7
Comment Human iPS cell line. HPS0002 and HPS0076 were derived from the same patient.Order Form ( C-0042, C-0034). Please see CiRA , Kyoto University if you are a researcher at a for–profit organization.
Animal human
Subspecies Caucasian
Sex Female
Age 36 years
Tissue skin
Exogene pMXs-Oct3/4, -Sox2, -Klf4, -c-Myc, pLenti6/UbC/V5-DEST, mouse Slc7a1
Morphology ES-like
Anchoraged Yes
Medium Primate ES Cell Medium+4ng/ml human basic FGF
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin+0.1% collagenase type IV+20% KSR+1mM CaCl2+PBS(-), 0.1% gelatin coated dish
Split ratio 1:3-6
Subculture frequency subculture : once/4-6days. medium change : every day
Cloned Yes
Lifespan infinite
Mycoplasma -
Chr distrb 46XX(20)
Short Tandem Repeat OK
memo DAP213 : Medium+2M DMSO+1M Acetamide+3M Propyleneglycol, feeder : SNL 76/7 (X-rays:5000R or MMC) 1.5-2.5x10^(6) cells/100mm dish
Originator Yamanaka, Shinya
Depositor http://www.cira.kyoto-u.ac.jp/
Restriction Attach TERMS and CONDITIONS to MTA (C-0034). Order Form ( C-0042, C-0034). f
Photo 2014-05-20
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank@brc.riken.jp) regarding any kind of for-profit use.


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