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CELL SEARCH SYSTEM

HPS0077  :  454E2
Comment Human iPS cell line. Application consideration. Order Form ( C-0042, C-0034).
Animal human
Subspecies Japanese
Sex Female
Age 16 years
Tissue dental pulp
Exogene pCXLE, human Oct3/4, human Sox2, human Klf4, human L-Myc, human Lin28, p53 shRNA
Morphology ES-like
Anchoraged Yes
Medium Primate ES Cell Medium+4ng/ml human basic FGF
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin+0.1% collagenase type IV+20% KSR+1mM CaCl2+PBS(-), 0.1% gelatin coated dish
Split ratio 1:3-5 split
Subculture frequency subculture : once/3-5days. medium change : every day
Cloned Yes
Lifespan infinite
Mycoplasma -
Chr distrb 46XX(20)
Short Tandem Repeat OK
memo DAP213 : Medium+2M DMSO+1M Acetamide+3M Propyleneglycol, feeder : SNL 76/7 (X-rays:5000R or MMC) 1.5-2.5x10^(6) cells/100mm dish
Originator Yamanaka, Shinya
Depositor http://www.cira.kyoto-u.ac.jp/
Restriction Please see CiRA , Kyoto University if you are a researcher at a for–profit organization.Attach TERMS and CONDITIONS to MTA (C-0034). f
Photo 2014-05-20
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank@brc.riken.jp) regarding any kind of for-profit use.


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