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RCB0009  :  PC-12
Comment Pheochromocytoma, differentiate by NGF stimulation
Animal rat
Tissue pheochromocytoma
Morphology chromaffin cell-like
Anchoraged Yes
Medium DMEM+10%FBS+10%HS
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin
Split ratio 1:8 split
Subculture frequency once/week
Cloned No
Lifespan infinite
Mycoplasma -
Isozyme analysis LD, NP, G6PD, AST, animal PCR
Chr distrb Lot22: 33-79(50):33(1),39(1),40(45),65(1),79(2)
Originator L. Green & A. Tischler
Depositor Noda, Makoto
Restriction Basically, there is no restriction regarding academic use. a
Reference 1078 1079 1318 1726
User's Publication 1646 1667 1972 2055 2056 2190 2191 2198 2367 2369 2641 2642 2647 2715 2716 2717 3007 3010 3015 3067 3068 3070 3163 3184 3186
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.

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