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CELL SEARCH SYSTEM

RCB0207  :  MRC-5 SV1 TG1
Comment SV40-transformed, 6TG-resistant and HAT-sensitive.
Animal human
Sex Male
Age fetus
Tissue lung
Morphology fibroblast-like
Anchoraged Yes
Medium HF-RITC80-7 (or MEM ALPHA)+10%FBS
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin
Split ratio 1:8 split
Subculture frequency once/week
Cloned No
Lifespan infinite
Mycoplasma -
Virus HIV-1(-)
Isozyme analysis LD, NP
Chr distrb 71-145(50):/74(3),75(3),76(8),77(12),78(10),79(4)/
Short Tandem Repeat OK
Originator Jacobs, P.
Depositor ECACC
Restriction Basically, there is no restriction regarding academic use. a
User's Publication 3378
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank@brc.riken.jp) regarding any kind of for-profit use.


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