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RCB0211  :  MRC-5
Comment Normal embryonic lung fibroblast.
Animal human
Sex Male
Age fetus
Tissue lung
Morphology fibroblast-like
Anchoraged Yes
Medium MEM ALPHA (orHF-RITC80-7) +10%FBS
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin
Split ratio 1:4 split
Subculture frequency twice/week
Cloned No
Lifespan finite
Mycoplasma -
Virus Lot.1: HIV-1(-)
Isozyme analysis Lot.13: LD, NP
Chr distrb Lot1:44-47(50):44(3),45(7),46(39),47(1)
Short Tandem Repeat OK
Originator Jacobs, J. P.
Depositor ECACC
Restriction Basically, there is no restriction regarding academic use. a
Reference 1028
User's Publication 1808 1809 2070 2631 2709 2892 2894 3202 3382 3413 3434 3579
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.

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