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RCB0218  :  MRC-5, known PDL
Comment Normal embryonic lung fibroblast with accurate PDL
Animal human
Sex Male
Age 14 weeks embryo
Tissue embryo/fetus, lung
Morphology fibroblast-like
Anchoraged Yes
Medium HF-RITC80-7 (or MEM ALPHA)+10%FBS
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin
Split ratio 1:4 split
Subculture frequency twice/week
Cloned No
Lifespan finite
Mycoplasma -
Virus HIV-1(-)
Isozyme analysis LD, NP
Chr distrb 42-47(50):42(3),43(3),44(6),45(12),46(24),47(2)
Short Tandem Repeat OK
Originator Jacobs, J. P.
Depositor Ohno, Tadao
Restriction Basically, there is no restriction regarding academic use. a
User's Publication 2672
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.

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