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RCB0247  :  10T1/2
Comment Clone 8, Contact-inhibited semi-normal
Animal mouse, C3H
Age embryo
Tissue embryo
Morphology fibroblast-like
Anchoraged Yes
Medium BME(2mM L-Glutamine)+10%FBS
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin
Split ratio 5x10^(4)cells/6cm dish
Subculture frequency once/week
Cloned No
Lifespan infinite
Mycoplasma -
Isozyme analysis Lot.20: LD, NP
Chr distrb Lot1:65-81(50):/76(1),77(3),78(5),79(12),80(25)/
Originator Reznikoff, Catherine A.
Depositor Terasima, Toyozo
Restriction Basically, there is no restriction regarding academic use. a
Reference 44 961 962 2000 2001
User's Publication 1329 2480 2635 2644 2874 2893 2899 2915 2936 3158 3159 3183 3220 3343 3357 3584
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.

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