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RCB0285  :  CHO-K1
Comment Widely used cell line, especially for biotechnol
Animal hamster, Chinese
Sex Female
Age adult
Tissue ovary
Morphology epithelial-like
Anchoraged Yes
Medium HamF12+10%FBS
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin
Split ratio 1:8 split
Subculture frequency twice/week
Cloned No
Lifespan infinite
Mycoplasma -
Isozyme analysis animal PCR (isozyme : not tested)
Chr distrb Lot.1:17-58(50): *see memo
memo *17(3),18(3),19(10),20(21),21(6),26(1)/
Originator Puck, T. T.
Depositor Seto, Toshio
Restriction Basically, there is no restriction regarding academic use. a
Reference 1068 2116 2586
User's Publication 2382 2651 2701 2893 3031 3032 3042 3060 3099 3100 3204 3226
Photo 2013-03-25
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.

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