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RCB0535  :  RAW 264
Comment Buck-up culture of ECA8510291 . Mouse leukemic monocyte. Abelson leukemia virus induced. Application consideration
Animal mouse, BALB/c
Tissue leukemic monocyte
Morphology macrophage-like
Anchoraged Yes
Medium MEM+10%FBS+0.1mM NEAA
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.05% trypsin+0.02% EDTA or 0.25% trypsin+0.02% EDTA
Split ratio 1:4-8 split
Subculture frequency 1-2 times/week
Cloned No
Lifespan infinite
Mycoplasma -
Isozyme analysis Lot.31: LD, NP
Chr distrb Lot1:39-160(50):39(5),40(36),41(1),78(1),80(3)
Depositor ECACC
Restriction Basically, there is no restriction regarding academic use. a
Reference 716
User's Publication 1645 2366 2423 2504 2654 2664 2688 2865 2890 2903 2904 2919 2922 2973 3098 3135 3160 3190 3193 3197 3200 3209 3210 3212 3220 3224 3225 3229 3232 3364 3365 3392 3578 3578
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank@brc.riken.jp) regarding any kind of for-profit use.


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