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RCB0557  :  B16 melanoma 4A5
Comment Melanin pigment producing mouse melanoma. Application consideration
Animal mouse, C57BL/6
Tissue skin
Morphology fibroblast-like
Anchoraged Yes
Medium DMEM+10%FBS
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin
Split ratio 1:8 split
Subculture frequency 1-2 times/week
Cloned Yes
Lifespan infinite
Mycoplasma -
Isozyme analysis LD, NP
Depositor Sugimoto, Yoshinori
Restriction Basically, there is no restriction regarding academic use. a
Reference 547 587 3488
User's Publication 2368 2846 3221 3313 3381
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.

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