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CELL SEARCH SYSTEM

RCB0698  :  HFSKF-II
Comment Normal human fetal skin fibroblast.
Animal human
Subspecies Japanese
Sex Unknown
Age 11 weeks embryo
Tissue embryo/fetus, skin
Morphology fibroblast-like
Anchoraged Yes
Medium HamF12+15%FBS
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin
Split ratio 1:4 split
Subculture frequency twice/week
Cloned No
Lifespan finite
Mycoplasma -
Virus HIV-1(-)
Isozyme analysis LD, NP
Chr distrb 45-46(50):45(2),46(48)
Short Tandem Repeat OK
Originator Ishiwata, Isamu & Ishikawa, Hiroshi
Depositor Ishiwata, Isamu
Restriction Basically, there is no restriction regarding academic use. a
User's Publication 2860
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank@brc.riken.jp) regarding any kind of for-profit use.


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