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RCB0991  :  G-361
Comment Human melanoma producing melanin up to 50 PDL. Backup of ECA88030401 .
Animal human
Sex Male
Age 31 years
Tissue skin
Morphology epithelial-like
Anchoraged Yes
Medium McCoy's 5A +10%FBS
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method pipetting
Split ratio 1:8 split
Subculture frequency once/week
Cloned No
Lifespan infinite
Mycoplasma -
Virus HIV-1(-)
Isozyme analysis LD, NP
Chr distrb 65-133(50):/67(3),68(5),69(13),70(7),71(6),72(4)/
Short Tandem Repeat OK
Depositor ECACC
Restriction Basically, there is no restriction regarding academic use. a
Reference 125
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.

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