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RCB0992  :  HOS
Comment Sensitive to further transformation with both virus and chemicals. Backup of ECA87070202 .
Animal human
Sex Female
Age 13 years
Tissue bone
Morphology epithelial-like
Anchoraged Yes
Medium MEM+10%FBS+0.1mM NEAA
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin
Split ratio 1:8-10 split
Subculture frequency twice/week
Cloned No
Lifespan infinite
Mycoplasma -
Virus HIV-1(-)
Isozyme analysis LD, NP
Chr distrb 47-76(50):47(5),48(15),49(20),50(9),76(1)
Short Tandem Repeat OK
Depositor ECACC
Restriction Basically, there is no restriction regarding academic use. a
Reference 1827 1828 1829
User's Publication 3041
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.

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