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RCB0995  :  MDCK
Comment Canine kidney epithelial. Sensitive to wide range of viruses. Backup of ECA84121903 .
Animal dog
Tissue kidney
Morphology epithelial-like
Anchoraged Yes
Medium MEM+10%FBS+0.1mM NEAA
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin or 0.05% trypsin+0.02% EDTA
Split ratio 1:8 split
Subculture frequency 1-2 times/week
Cloned No
Lifespan infinite
Mycoplasma -
Isozyme analysis LD, NP, animal PCR
Originator Madin, S. H. & Darby, N. B.
Depositor ECACC
Restriction Basically, there is no restriction regarding academic use. a
Reference 258 2581
User's Publication 2485 2636 3080 3219
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.

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