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RCB1005  :  KP4
Comment Pancreatic ductal cell carcinoma. Mother cell line of KP4-1, -2, and -3.
Animal human
Subspecies Japanese
Sex Male
Age 50 years
Tissue pancreas, ascites meta
Morphology epithelial-like
Anchoraged Yes
Medium (DMEM:HamF12=1:1)+10%FBS
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin
Split ratio 1:8 split
Subculture frequency 1-2 times/week
Cloned Yes
Lifespan infinite
Mycoplasma -
Isozyme analysis LD, NP
Chr distrb 62-174(50):/64(6),65(6),67(8),69(6),70(5),71(2)/
Short Tandem Repeat OK
memo Mycoplasma was eliminated with MC-210 by the depositor.
Originator Nawata, H. & Nishi, Y.
Depositor Nishi, Y. & Nawata, H.
Restriction Basically, there is no restriction regarding academic use. a
Reference 1840
User's Publication 3106
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.

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