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CELL SEARCH SYSTEM

RCB1124  :  OP9
Comment Support hematpoietic stem cell and ES cell differentiation to blood cells and lymphocytes.
Animal mouse, B6C3Fe-a/a, op
Sex Unknown
Age 0 day
Tissue newborn, calvaria
Morphology fibroblast-like
Anchoraged Yes
Medium MEM ALPHA+20%FBS
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.05% trypsin+0.02% EDTA or 0.25% trypsin+0.02% EDTA
Split ratio 1:4 split
Subculture frequency MC or SC once/2days
Cloned Yes
Lifespan infinite
Mycoplasma -
Isozyme analysis LD, NP
Originator Kodama, Hiroaki
Depositor Kodama, H. & Nakano, T.
Restriction Basically, there is no restriction regarding academic use. a
User's Publication 2428 2666 3171 3220 3357 3358 3411
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank@brc.riken.jp) regarding any kind of for-profit use.


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RIKEN BioResource Research Center