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RCB1366  :  HuH-7
Comment Said producing alpha-fetoprotein, alpha-antitrypsin, ceruloplasmin, fibrinogen, fibronectin, etc.
Animal human
Subspecies Japanese
Sex Male
Age 57 years
Tissue liver
Morphology epithelial-like
Anchoraged Yes
Medium DMEM+10%FBS
Antibiotics 100U/ml Penicillin + 100μg/ml Streptmycin
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin
Split ratio 1:8 split
Subculture frequency 1-2 times/week
Cloned No
Lifespan infinite
Mycoplasma -
Virus Lot.1: HBV(-), HCV(-)
Isozyme analysis LD, NP, PepB, MPI
Originator Nakabayashi, Hidekazu
Depositor Huh, Nam, Ho
Approver Sakaguchi, Masakiyo Sakaguchi, Masakiyo
Restriction A prior written permission of the approver is necessary for any kind of use including academic use and for-profit use. b
Reference 135 1744 1859 2578
User's Publication 3182 3270 3342 3471
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.

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RIKEN BioResource Research Center