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RCB1549  :  YSCCC
Comment Human cell line derived from cholangiocellular carcinoma. Cell growth is slow.
Animal human
Subspecies Japanese
Sex Female
Age 70 years
Tissue liver, ascites meta
Morphology lymphocyte-like
Anchoraged Yes
Medium RPMI1640+10%FBS
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin
Split ratio 1:4 split
Subculture frequency once/2weeks
Cloned No
Lifespan infinite
Mycoplasma -
Virus HBV(-), HCV(-)
Isozyme analysis LD, NP
Chr distrb 65-73(50):/67(5),68(8),69(10),70(11),71(8),72(4)/
Short Tandem Repeat OK
Originator Imanishi, Hiroyasu
Depositor Imanishi, Hiroyasu
Restriction Basically, there is no restriction regarding academic use. a
Photo 2006-06-02
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.

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