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RCB1637  :  293
Comment Sheared human Ad5 DNA-transformed cell line. Widely used for production of manipulated adenovirus. Back up culture of ECA2737.
Animal human
Age embryo
Tissue kidney
Morphology epithelial-like
Anchoraged Yes
Medium MEM+10%FBS+0.1mM NEAA
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin
Split ratio 1:8 split
Subculture frequency once/week
Cloned No
Lifespan infinite
Mycoplasma -
Isozyme analysis LD, NP
Chr distrb Lot1:63-71(50):/65(5),66(5),67(8),68(10),69(10)
Short Tandem Repeat OK
Depositor ECACC
Restriction Basically, there is no restriction regarding academic use. a
Reference 2414 2415 3175 3489 3490 3491
User's Publication 2993 3350 3431
Photo 2006-06-02
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.

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