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RCB1862  :  NIH3T3-3-4
Comment Subclone of NIH3T3
Animal mouse, NIH Swiss
Age embryo
Tissue embryo
Morphology fibroblast-like
Anchoraged Yes
Medium DMEM+10%CS
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin
Split ratio 3x10^(5)cells/6cm dish
Subculture frequency once/3 or 4 days
Cloned Yes
Previous name NIH3T3-3 (RCB0150)
Lifespan infinite
Mycoplasma -
Isozyme analysis LD, NP
Originator Todaro, G. J.
Depositor Ohno, Tadao
Restriction Basically, there is no restriction regarding academic use. a
Reference 298 595 596 787 963 1404 1819
User's Publication 1943 2057 2193 2421 2480 2486 2637 2646 3385
Photo 2008-07-18
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.

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