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CELL SEARCH SYSTEM

RCB1904  :  MCF7
Comment Human cell line derived from breast adenocarcinoma. TKG0479 (Deposited from Tohoku Univ.).
Animal human
Subspecies Caucasian
Sex Female
Age 69 years
Tissue brest, pleural fluid
Morphology epithelial-like
Anchoraged Yes
Medium MEM+10%FBS+0.1mM NEAA+1mM Sodium Pyruvate
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin
Split ratio 1:8 split
Subculture frequency once/week
Cloned No
Lifespan infinite
Mycoplasma -
Isozyme analysis LD, NP, AST
Short Tandem Repeat OK
memo Mycoplasma was eliminated with BM Cycline and MC-210 by the depositor.
Originator Soule H.D.
Depositor Obinata, Masuo
Restriction Basically, there is no restriction regarding academic use. a
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank@brc.riken.jp) regarding any kind of for-profit use.


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