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RCB1907  :  TKKK
Comment Human cell line derived from intrahepatic bile duct cancer. TKG0456 (Deposited from Tohoku Univ.).
Animal human
Tissue Intrahepatic bile duct
Morphology epithelial-like
Anchoraged Yes
Medium DMEM+10%FBS
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.05% trypsin+0.02% EDTA or 0.25% trypsin+0.02% EDTA , type I collagen coated dish
Split ratio 1:4 split
Subculture frequency once/ 4 weeks
Cloned No
Lifespan infinite
Mycoplasma -
Virus HBV(-), HCV(-)
Isozyme analysis LD, NP
Short Tandem Repeat OK
Originator Katayose, Y.
Depositor Obinata, Masuo
Restriction Basically, there is no restriction regarding academic use. a
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.

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