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RCB1941  :  Li-7
Comment Human hepatoma cell line. This cell line was established from nude mouse xenograft tumor (Dr. Hirohashi., National Cancer Center, Tokyo) by Tanno, H. (Tohoku Univ., 1st Surg). AFP producing. TKG0368 (Deposited from Tohoku Univ.).
Animal human
Sex Male
Age 45 years
Tissue liver
Morphology epithelial-like
Anchoraged Yes
Medium RPMI1640+10%FBS
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.05% trypsin+0.02% EDTA or 0.25% trypsin+0.02% EDTA
Split ratio 1:8 split
Subculture frequency once/week
Cloned No
Lifespan infinite
Mycoplasma -
Virus HBV(-), HCV(-)
Isozyme analysis LD, NP
Chr distrb 57-64(50):57(8),58(9),59(11),60(2),61(3),62(12)/
Short Tandem Repeat OK
Originator Tanno, H. (Tohoku Univ., 1st Surg)
Depositor Obinata, Masuo
Restriction Basically, there is no restriction regarding academic use. a
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.

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