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RCB1943  :  HuH-28
Comment Human cell line derived from cholangiocellular carcinoma. TKG0438 (Deposited from Tohoku Univ.).
Animal human
Tissue liver
Morphology spindle-shaped
Anchoraged Yes
Medium MEM+10%FBS
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin+0.02% EDTA
Split ratio 1:4 split
Subculture frequency once/2 weeks
Cloned No
Lifespan infinite
Mycoplasma -
Virus HBV(-), HCV(-)
Isozyme analysis LD, NP
Short Tandem Repeat OK
Originator Kusaka, Y.
Depositor Obinata, Masuo
Restriction Basically, there is no restriction regarding academic use. a
Photo 2008-01-18
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.

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