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RCB2202  :  293T
Comment Human cell line expressing SV40 large T antigen abundantly.
Animal human
Age embryo
Tissue kidney
Morphology epithelial-like
Anchoraged Yes
Medium DMEM+10%FBS (heat inactivated)
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin
Split ratio 1:10 split
Subculture frequency twice/week
Cloned No
Lifespan infinite
Mycoplasma -
Isozyme analysis Lot.19:animal PCR (isozyme : not tested)
Short Tandem Repeat OK
Depositor Miyoshi, Hiroyuki
Restriction Basically, there is no restriction regarding academic use. a
Reference 3489 3490 3491
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.

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