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CELL SEARCH SYSTEM

RCB2403  :  MG6
Comment Mouse microglial cell line immortalized by a replication-deficient retroviral vector containing human c-myc gene. No significant production of infectious viral particles from MG6 cells was confirmed by bioassay using SC-1 cells.
Animal mouse, C57BL/6
Sex Unknown
Age 1 day
Tissue brain
Exogene human c-myc, neomycin, retroviral vector
Morphology macrophage-like
Anchoraged Yes
Medium DMEM(high glucose)+10%FBS+10μg/ml Insuline+0.1mM 2-Mercaptoethanol
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method pipetting, non-tissue culture dish
Split ratio 1:8 split
Subculture frequency once/4-7 days
Cloned Yes
Lifespan infinite
Mycoplasma -
Isozyme analysis LD, NP
Originator Kitani, Hiroshi
Depositor Kitani, Hiroshi
Approver Kitani, Hiroshi Kitani, Hiroshi
Restriction 1) A permission of the approver is required. 2)If any intellectual Property is generated during the use of the delivered Material, the Recipient shall consult with the Administrator of the Material at the NIAS. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Neurosci Lett 2006;407:205-210, Biochim Biophys Acta 2005;1726:177-186 ) designated by the DEPOSITOR is required. b
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank@brc.riken.jp) regarding any kind of for-profit use.


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