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RCB2863  :  N14.5
Comment Rat neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus
Animal rat, Wister (Transgenic rat)
Sex unknown
Age 3 months
Tissue brain
Exogene tsA58mutant
Morphology fibroblast-like
Anchoraged Yes
Medium Neurobasal Medium (NBM)+5%FBS+2%B27+0.5mM L-glutamine+20ng/ml EGF+20ng/ml bFGF
Antibiotics Free
Growth temp 33oC
CO2 concentration 5%
Passage method 0.25% trypsin, type I collagen coated dish
Split ratio 1:8 split
Subculture frequency once/week
Cloned Yes
Lifespan infinite
Mycoplasma -
Isozyme analysis animal PCR (isozyme : not tested)
Originator Matsushita, Tomoko
Depositor Hashimoto, Seiichi
Restriction In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Neuroscience. 2006;140:849-56) designated by the DEPOSITOR is required. f
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.

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