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RCB2867  :  HeLa/Fucci2
Comment A subline of the HeLa cell line expressing Fucci2, a cell cycle marker. Cell line name : HeLa/Fucci2 #3. Important notice regarding nomenclature of Fucci.
Animal human
Sex Female
Tissue cervix
Exogene Fucci2
Morphology epithelial-like
Anchoraged Yes
Medium DMEM+10%FBS
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin
Split ratio 1:8 split
Subculture frequency twice/week
Cloned Yes
Lifespan infinite
Mycoplasma -
Isozyme analysis LD, NP
Short Tandem Repeat OK
memo Cell name was changed. (HeLa.S-Fucci2 -> HeLa/Fucci2)
Originator Miyawaki, Atsushi
Depositor Miyawaki, Atsushi
Approver Miyawaki, Atsushi Miyawaki, Atsushi
Restriction 1) The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2) The RECIPIENT should obtain written approval from the UCSD using the [mCherry FORM]. 3) A RECIPIENT who belongs to a non-profit organization may use the¡¡BIOLOGICAL RESOURCE for an academic research that is non-commercial. 4) In publishing the research results related to the BIOLOGICAL RESOURCE¡¤a citation of the following literature is necessary. Asako Sakaue-Sawano, Tamiyo Kobayashi, Kenji Ohtawa and Atsushi Miyawaki. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol.12:2. 5) The RECIPIENT agrees to acknowledge the DEPOSITOR in any publication, oral or written, reporting use of the BIOLOGICAL RESOURCE and provide a copy of the publication to the DEPOSITOR. b, mCherry
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.

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