DNA RESULT RIKEN BioResource Center
May. 22, 2014
RDB01851
Type DNA clone
Name of clone pC
Alternative name Tobacco cationic preoxidase isozyme 40 K
Description The clone is encoded tobacco cationic preoxidase isozyme 40 kD from cultured tobacco cell BY2. It is possible to excise the insert DNA with restriction enzymes of EcoRI and NotI.
Type of inserted Nicotiana tabacum cDNA ()
Size of inserted 1,224 bp
Classification Plasmid
Restriction site BamH I - 0.76, 3.4 kb, EcoR V - 0.18, 0.86, 3.2 kb, EcoR I - 1.2, 3.0 kb, Not I - 0.04, 1.2, 2.9 kb, Pvu II - 1.7, 2.5 kb, Xba I - 0.7, 3.5 kb
Gene Cationic peroxidase
Locus Link
UniGene
Ref Seq
DDBJ D42065
Gene ID
Vector pBluescript SK (-)
Size of vector 3.0 kb
Cloning site EcoR I
Genetic markers Amp^r
Host E. coli
Host strain XL1-Blue
Keywords cationic peroxidase, isozyme, 40 kDa, cDNA, cultured tobacco cells BY2, cell wall metabolism, Nicotina tobacco L. cv. Bright Yellow 2; _plant
Growth condition 37 C, LB+Amp
Reference Eur J Biochem. 1995 Mar 15;228(3):855-62.  
Plant Physiol. 1996 Feb;110(2):713-4.  
Parent Virus
Relation
Developer Matsumoto, Shinya (Kyoto University)  
Depositor
Sasaki, Ryuzo (Kyoto University)  
  Sasaki, Ryuzo 
Restrict for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. j
Remarks
Restriction map  gif
Nucleotide sequence 1
MS using RDB clone   

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