The Gene-trap Mouse ES cell clones

The Gene-trap Mouse ES cell clones

The Gene-trap Mouse ES cell clones using UPATrap method developed by Dr. Yasumasa Ishida (Nara Institute of Science and Technology) have been deposited. These cells can be used in the in vitro differentiation study. In addition, gene inactivated mouse line can be generated by using these cell clones.

After accepting the order, we will start the culture of the cell clone. Thus, approximately three months will be required to prepare the cell clone for provision. Thank you in advance for your understanding and cooperation.

·	ES cell: (C57BL/6J x 129/Sv^Jae)F1 embryonic stem cell
·	Terms and conditions of use
	(1)	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. When the RECIPIENT use the BIOLOGICAL RESOURCE in collaboration with a for-profit organization, it is recognized as a for-profit utilization and the RECIPIENT must reach agreement on terms and conditions of use of it with the DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
	(2)	For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with the DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
	(3)	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR (Dr. Yasumasa Ishida ) is requested.
	(4)	In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of a paper (Nucleic Acids Res. 2005;33:e20) is requested.
	(5)	The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
	(6)	When the RECIPIENT established a mouse line by using of the BIOLOGICAL RESOURCE, the RECIPIENT must deposit the mouse line to the Experimental Animal Division of RIKEN BioResource Center.
·	Culture medium:DMEM(high glucose, Sodium pyruvate(-)) +15%FBS+0.1mM NEAA +0.1mM 2-Mercaptoethanol +1000U/ml LIF Feeder:SNL cells(SNL 76/7)
·	How to request cellqa@brc.riken.jp
·	The NAISTrap Project
·	International Gene Trap Consortium (IGTC)
·	NCBI GSS Search

	List of the ES cell clones

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