Strain Data Sheet
Data update: Jul 19, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
RBRC No. RBRC00153  
Type Transgene CongenicCartagena   4wks,♀
Species Mus musculus  
Strain name B6.Cg-Tg(Camk2a-cre)10Kmik/KmikRbrc  
Former Common name C57BL/6-TgN(a-CaMKII-nlCre)/10  
H-2 Haplotype No Data  
Background strain C57BL/6JJcl  
 1 Appearance black  
Genotype No Data  
Strain development Deposited by Dr. Katsuhiko Mikoshiba, Brain Science Institute, RIKEN.  
Strain description The Cre-recombinase activity was detected specifically in the nuclear fraction of the cerebrum. The expression level of the cre gene in each line of Tg mice was following order; Line 10, 26>22, 17N, 11, 20. The expression of the cre was detected by RT-PCR (including semi-quantitative one), Western blotting and the Cre-recombinase activity assay. There are some variation in the amount of expression in the cerebrum and leaky expression (less than 1/10 to 1/100 of that in the cerebrum at maximum) in the cerebellum among the different lines.  
Colony maintenance Sibling mating (Hemizygote x Wild-type)
Reproductive perdormance is good.  
Health Report
Gene Details
Promoter No Data  
 1 Symbol nls  
Symbol name SV40 large T antigen nuclear localization signal (NLS)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter mouse Camk2a promoter  
 2 Symbol cre  
Symbol name Phage P1 Cre recombinase  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References No Data  
Research applications Cre/loxP system,
Neurobiology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT of BIOLOGICAL RESOURCE shall prior contact with the DEPOSITOR, discussn use of it and obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and NOT for commercial use. The RECIPIENT can use the BIOLOGICAL RESOURCE in a collaboration basis with the DEPOSITOR, except for some cases where only ACKNOLEGEMENT is requested by citing the DEPOSITOR. BEFORE the publication on the BIOLOGICAL RESOURCE by the DEPOSITOR, the RECIPIENT agrees to use the BIOLOGICAL RESOURCE in collaboration with the DEPOSITOR and add Drs. Akira Futatsugi and Katsuhiko Mikoshiba as a co-authors. The RECIPIENT agrees to cite publications requested by the DEPOSITOR.  
Additional information
 1 Lab HP (Japanese)  
 2 Genotyping protocol <PCR>  
Depositor Mikoshiba, Katsuhiko (RIKEN Center for Brain Science)  Mikoshiba, Katsuhiko 
Strain Status /
(Expected delivery)

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