Strain Information | |
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Image | |
BRC No. | RBRC00169 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6.Cg-Tnc<tm1Sia>/Rbrc |
Former Common name | C57BL/6N-TgH(Tnc), B6-TN, TNKO |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | C57BL/6NJcl |
Appearance | black [a/a, B/B, C/C] |
Strain development | Congenic strains were developed by Dr. M. Kusakabe, Cell Biology Laboratory, Life Science Tsukuba Research Center, RIKEN since 1992. A lacZ gene and neomycin resistant cassette was transfered into TT2 ES cells to replace the exon 2, just before the translation initiation codon of the Tnc gene. This strain was backcrossed to C57BL/6N. |
Strain description | Tenascin C knock-out mice. This strain is useful for studying the biological function of extracellular matrix tenascin C in vivo, especially in the field of tumor development, tumor metastasis, wound healing, tissue regeneration, angiogenesis, tissue interaction etc. The tenascin C deficient congenic strains were developed by successive backcrossing with A/J (RBRC00007), BALB/cA (RBRC00067), C57BL/6N (RBRC00169), C3H/HeN (RBRC00110) and GRS/A (RBRC00193). |
Colony maintenance | Homozygote x HomozygoteHomozygous mutant mice are viable and fertile. |
References | J. Neurosci., 15, 1971-1983 (1995). 7534342 Biochem. Biophys. Res. Commun., 221, 151-156 (1996). 8660327 Biochem. Biophys. Res. Commun., 231, 356-359 (1997). 9070278 Biochem. Biophys. Res. Commun., 290, 1220-1227 (2002). 11811993 J. Comp. Neurol., 430, 485-500 (2001). 11169482 Invest. Ophthalmol. Visual. Sci., 40, 1071-1080 (1999). 10235540 Perspect. Dev. Neurobiol., 2, 111-116 (1994). 7530137 Genes Dev., 6, 1821-1831 (1992). 1383086 Gene, 104, 177-185 (1991). 1717349 Jpn. J. Cancer Res., 93, 968-975 (2002). 12359049 J. Invest. Dermatol., 111, 930-935 (1998). 9856798 Exp. Mol. Pathol., 72, 186-195 (2002). 12009782 Am. J. Pathol., 152, 1237-1245 (1998). 9588892 |
Health Report | |
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Examination Date / Room / Rack | 2022/04/25Room:3-CRack:K |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Tnctenascin C4Tnc<tm1Sia>targeted mutation 1, Shinichi AizawaTnc; tenascin C, Hxb, Ten, TN-C, AI528729 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)4 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)4neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | E. coli lacZ, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse tenascin genomic DNA |
Research application | Neurobiology Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Am. J. Pathol., 152, 1237-1245 (1998). J. Invest. Dermatol., 111, 930-935 (1998). Invest. Ophthalmol. Visual. Sci., 1071-1080 (1999). |
Depositor | RIKEN BRC (RIKEN BRC) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Genetic Background |
BRC mice in Publications |
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Nakamura-Ishizu A, Okuno Y, Omatsu Y, Okabe K, Morimoto J, Uede T, Nagasawa T, Suda T, Kubota Y. Extracellular matrix protein tenascin-C is required in the bone marrow microenvironment primed for hematopoietic regeneration. Blood 119(23) 5429-37(2012) 22553313 Xu M, Ye Z, Zhao X, Guo H, Gong X, Huang R. Deficiency of tenascin-C attenuated cardiac injury by inactivating TLR4/NLRP3/caspase-1 pathway after myocardial infarction. Cell Signal 86 110084(2021) 34271086 Imanaka-Yoshida K, Matsumoto K, Hara M, Sakakura T, Yoshida T. The dynamic expression of tenascin-C and tenascin-X during early heart development in the mouse. Differentiation 71(4-5) 291-8(2003) 12823230 Matsumoto K, Takahashi K, Yoshiki A, Kusakabe M, Ariga H. Invasion of melanoma in double knockout mice lacking tenascin-X and tenascin-C. Jpn J Cancer Res 93(9) 968-75(2002) 12359049 Ito K, Morimoto J, Kihara A, Matsui Y, Kurotaki D, Kanayama M, Simmons S, Ishii M, Sheppard D, Takaoka A, Uede T. Integrin α9 on lymphatic endothelial cells regulates lymphocyte egress. Proc Natl Acad Sci U S A 111(8) 3080-5(2014) 24516133 Kubo Y, Ishikawa K, Mori K, Kobayashi Y, Nakama T, Arima M, Nakao S, Hisatomi T, Haruta M, Sonoda KH, Yoshida S. Periostin and tenascin-C interaction promotes angiogenesis in ischemic proliferative retinopathy. Sci Rep 10(1) 9299(2020) 32518264 |