Strain Data Sheet
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Data update: Jun 20, 2019
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RBRC No. RBRC00235  
Type Targeted Mutation CongenicCartagena   8wks,♂
Species Mus musculus  
Strain name B6.129X1-Chrng<tm1Mtak>/10Rbrc  
Former Common name Achr, γ  
H-2 Haplotype No Data  
ES cell line RW4 [129X1/SvJ]  
Donor strain 129X1/SvJ via RW4 ES cell line  
Background strain C57BL/6JJcl  
Appearance
 1 Appearance black  
Genotype No Data  
Strain development Developed by Dr. Masazumi Takahashi. This strain has been introduced into C57BL/6 genetic background by successive backcrossing for more than 10 generations in RIKEN Tsukuba Institute.  
Strain description The acetylcholine receptors are known to have important roles in neuro-muscular functions. The gamma subunit are expressed in embryonic stages and the epsilon subunit are expressed postnatal stages.
The acetylcholine receptors gamma and epsilon subunits have been disrupted by homologous recombination to study the functions of these genes and switching mechanism of gamma to epsilon subunits. The gamma subunit deficient homozygotes died after two days post delivery, suffering from inability of motor function of the limbs and drinking milk. The analysis on the neuro-muscular junctions showed the structural and electrophysiological abnormalities.  
Colony maintenance Backcrossing to C57BL/6. Or sister-brother mating of heterozygotes. Breeding performance is good.  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Chrng  
Symbol name cholinergic receptor, nicotinic, gamma polypeptide  
Chromosome 1  
Common name Achr-3, Acrg  
Symbol description No Data  
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)  
 2 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 1  
Common name neo; neomycin;  
Symbol description No Data  
References Development. 1997 Dec;124(24):5075-86.
EMBO Rep. 2002 Jul;3(7):674-81.
FEBS Lett. 1987 Oct 19;223(1):104-12.
Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):13286-91.
Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13413-8.  





Research applications Neurobiology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Takahashi, Masazumi (Centre National de Genotypage)  Takahashi, Masazumi 
Strain Status /
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