Strain Data Sheet
Data update: Jul 19, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
RBRC No. RBRC00267  
Type TransgeneCartagena   8wks,♀
Species Mus musculus  
Strain name C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb  
Former Common name C57BL/6-TgN(act-EGFP)OsbC14-Y01-FM131  
H-2 Haplotype No Data  
Background strain Inter- Substrain hybrid between C57BL/6Cr Slc and C57BL/6J Jcl  
 1 Appearance black  
Genotype a/a  
Strain development Developed by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs. C57BL/6 background (C57BL/6JJcl and C57BL/6NCrSlc mixed).  
Strain description These transgenic mice carry an EGFP (enhanced green fluorescence protein) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer. Both homozygous and hemizygous transgenic mice express GFP in all over the body. This strain is useful for detection of cell fate in transplantation and developmental studies. Homozygous transgenic females experienced show a high incidence of sudden death (about 30%), putatively due to dilated cardiomyopaty. Transgene integration site: 14D1.  
Colony maintenance Carrier x Carrier (Homozygote x Homozygote) [or Crossing to C57BL/6NCrSlc]  
Health Report
Gene Details
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)  
 1 Symbol GFP  
Symbol name Green Fluorescent Protein (Jellyfish)  
Chromosome 14  
Common name No Data  
Symbol description No Data  
References Curr Top Dev Biol. 1999;44:1-20.
FEBS Lett. 1997 May 5;407(3):313-9.
Genomics. 2002 Dec;80(6):564-74.
Mol Reprod Dev. 1999 Sep;54(1):43-8.  

Research applications Cell Biology Research,
Developmental Biology Research,
Fluorescent Proteins/lacZ System,
Reproductive Biology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
FEBS Lett., 407, 313-319 (1997).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University ( The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP  
 2 Mouse of the Month Feb 2012
Genetic Background  
 3 Genotyping protocol <PCR>  
Depositor Okabe, Masaru (Osaka university)  Okabe, Masaru 
Strain Status /
(Expected delivery)

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