Strain Data Sheet
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Data update: Jul 19, 2019
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RBRC No. RBRC00305  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.129S-Tnf<tm1Ljo> Rela<tm1Yuo>/Rbrc  
Former Common name C57BL/6-Tnf<tm1Ljo> Rela<tm1Yuo>/Rbrc, TNFκB  
H-2 Haplotype No Data  
ES cell line W9.5/D3 for TNF alpha, CCE [129/Sv] for relA  
Donor strain RelA: 129S6/SvEvTac via EK.CCE ES cell line, THF: 129S1/Sv-p+ Tyr+ Kitl+ via W9.5 ES cell line  
Background strain C57BL/6  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Produced by corssing relA deficient mice and TNF deficient mice strains. Neomycin cassettes were inserted into the nucleotides 3704-5364 of TNF gene and the downstream of the translation initiation codon of relA gene, respectively. RelA deficient mice were backcrossed to C57BL/6 for 10 generations, and TNF deficient mice were backcrossed to C57BL/6 for 5 generations. Then, the two mutant strains were crossed to create the TNFkB mice. Developed by Takahiro Doi at Aichi Cancer Research Center in 1997.  
Strain description TNF and RelA double knockout mice. TNF, tumor necrosis factor is a multifunction proinflammatory cytokine. RelA, v-rel reticuloendotheliosis viral oncogene homolog A (Avian) is one of NF-kB family relating to the development of lymphocytes, suggested to be highly sensitive to TNF cytotoxicity. Mice lacking only relA gene die between days 14 and 15 of embryogenesis due to liver cell apoptosis by endogenous TNF cytotoxicity. Meanwhile, TNF/relA double-deficient mice are viable and have normal liver. This strain is useful for studies of functions of RelA in vivo.  
Colony maintenance TNF: Homozygote x Homozygote; RelA: Heterozygote x Wild-type  
Health Report
BRC facility Health Report in the last year and a half

Examination Date Room Rack
May 7, 2012 2-検 M
Mar 5, 2012 2-検 M
Jan 10, 2012 2-検 M
Nov 7, 2011 2-検 M
Sep 5, 2011 2-検 M
Jul 4, 2011 2-検 M
Apr 25, 2011 2-検 M
Mar 7, 2011 2-検 M
Gene Details
Promoter No Data  
 1 Symbol Tnf  
Symbol name tumor necrosis factor  
Chromosome 17  
Common name tumor necrosis factor alpha, DIF, Tnfa, TNFSF2, TNFalpha, TNF-alpha, TNF alpha, Tnfsf1a, tumor necrosis factor-alpha  
Symbol description No Data  
 2 Symbol Rela  
Symbol name v-rel reticuloendotheliosis viral oncogene homolog A  
Chromosome 19  
Common name p65 NFkB, p65 NF-kappa B, avian reticuloendotheliosis viral (v-rel) oncogene homolog A  
Symbol description No Data  
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)  
 3 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 19  
Common name neo; neomycin;  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 4 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 17  
Common name neo; neomycin;  
Symbol description No Data  
References J Exp Med. 1997 Mar 3;185(5):953-61.
Proc Natl Acad Sci U S A. 1997 Jul 22;94(15):8093-8.
Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):2994-9.  
NF-kappa B RelA-deficient lymphocytes: normal development of T cells and B cells, impaired production of IgA and IgG1 and reduced proliferative responses.
NF-kappa B RelA-deficient lymphocytes: normal development of T cells and B cells, impaired production of IgA and IgG1 and reduced proliferative responses.
NF-kappa B RelA-deficient lymphocytes: normal development of T cells and B cells, impaired production of IgA and IgG1 and reduced proliferative responses.
NF-kappa B RelA-deficient lymphocytes: normal development of T cells and B cells, impaired production of IgA and IgG1 and reduced proliferative responses.
Research applications Apoptosis Research,
Cancer Research,
Cardiovascular Research,
Developmental Biology Research,
Diabetes and Obesity Research,
Endocrine Deficiency Research,
Hematological Research,
Immunology and Inflammation Research,
Immunology and Inflammation Research,
Internal/Organ Research,
Neurobiology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Proc Natl Acad Sci USA., 96, 2994-2999 (1999).
The for-profit institutions should obtain written confirmation from Ludwig Institute for Cancer Research. Ludwig Institute for Cancer Research: 1275 York Avenue, New York NY 10021, USA, FAX: 1+212-717-3100
Any patent rights and other intellectual properties are retained by the depositor and Ludwig Institute for Cancer Research. Users should contact the depositor and Ludwig Institute for Cancer Research in the case of application for any patents with the results from these mice.  
Additional information
 1 Mouse of the Month May 2009  
 2 Genotyping protocol <PCR>  
Depositor Doi, Takahiro (RIKEN, BioResource Center)  Doi, Takahiro 
Strain Status /
Availability
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