Strain Data Sheet
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Data update: May 20, 2019
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RBRC No. RBRC00344  
Type TransgeneCartagena   8wks,♂
Species Mus musculus  
Strain name B6D2-Tg(CAG-AR*97Q)7-8Sobu  
Former Common name B6D2-TgN(hAndR/CAGn)/97Q-7-8-917  
H-2 Haplotype No Data  
Background strain Jcl:B6D2F1  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development The mice was generated by injecting AR-97Q construct into BDF1 fertilized eggs. The AR-97Q construct consisted of Chicken-actin promoter and human AR fragment harboring 97 CAGs. The founder Tg mice were backcrossed to C57BL/6J.  
Strain description Spinal and bulbar muscular atrophy (SBMA) is a polyglutamine disease with expansion of a CAG repeat in the androgen receptor (AR) gene. This transgenic mice serves as a SBMA model carrying a full-length AR containing 97 CAGs. The mice showed progressive muscular atrophy and weakness. The mice also showed diffuse nuclear staining and nuclear inclusions. These phenotypes were significant in male transgenic mice, and rescued by castration. Female transgenic mice showed much limited phenotypes which became more pronounced by administration of testosterone.  
Colony maintenance Survival and reproduction are reduced by extensive backcross to B6. Maintenance in the BDF1 background may improve the survival of the Tg.  
Health Report No Data  
Gene Details
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)  
 1 Symbol AR  
Symbol name androgen receptor (human)  
Chromosome UN  
Common name androgen receptor  
Symbol description No Data  
References Neuron. 2002 Aug 29;35(5):843-54.  








Research applications No Data  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Neuron, 35, 843-854 (2002).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval form the DEPOSITOR using the Approval Form. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Sobue, Gen (Nagoya University Graduate School of Medicine)  Sobue, Gen 
Strain Status /
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