Strain Data Sheet
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Data update: Jul 19, 2019
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RBRC No. RBRC00465  
Type TransgeneCartagena   wks
Species Mus musculus  
Strain name B6.Cg-Tg(Ext2,LacZ)E22Tsh/ShsRbrc  
Former Common name Tg-EXT2(E22), EXT2 transgenic mice (E22)  
H-2 Haplotype No Data  
Background strain C57BL/6JJcl  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Drs. K. Morimoto and T. Shirasawa at Tokyo Metropolitan Institute of Gerontology in 2001. The Ext2/lacZ plasmid was injected into the pronuclei of B6D2F1 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J.  
Strain description Ext2, exostoses (multiple) 2 encodes an essential component of the glycosyltransferase complex that is required for the biosynthesis of heparan sulfate. This transgenic mice express exogenous Ext2 in developing chondrocytes. The expression of Ext2 is histologically monitored by lacZ expression. The biosynthesis of heparan sulfate and the formation of trabeculae of Tg mice are significantly increased at 8 weeks of age. This strain is useful for studies of biosynthesis of heparan sulfate in vivo.  
Colony maintenance Backcross to C57BL/6 (Hemizygote x C57BL/6J)  
Health Report No Data  
Gene Details
Promoter mouse Col11a2 promoter  
 1 Symbol Ext2  
Symbol name exostoses (multiple) 2  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 2 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome UN  
Common name No Data  
Symbol description E. coli beta galactosidase  
References Biochem Biophys Res Commun. 2001 Aug 24;286(3):534-40.
Biochem Biophys Res Commun. 2002 Apr 12;292(4):999-1009.  
Mouse coq7/clk-1 orthologue rescued slowed rhythmic behavior and extended life span of clk-1 longevity mutant in Caenorhabditis elegans.
Mouse coq7/clk-1 orthologue rescued slowed rhythmic behavior and extended life span of clk-1 longevity mutant in Caenorhabditis elegans.
Research applications Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The DEPOSITOR should be a co-author in articles when the RECIPIENTs publish their data using these mice for 2 year after provided to the RIKEN BRC.
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.  
Additional information
 1 Lab HP (Japanese)  
 2 Genotyping protocol <PCR>  
Depositor Shirasawa, Takuji (Juntendo University)  Shirasawa, Takuji 
Strain Status /
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