Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2019
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RBRC No. RBRC00522  
Type Targeted Mutation CongenicCartagena   18wks,♂
Species Mus musculus  
Strain name C57BL/6-Rag2tm1Fwa-IL2rbtm1Mak  
Former Common name C57BL/6-Rag2tm1Fwa-IL2rbtm1Och  
H-2 Haplotype No Data  
Background strain No Data  
Appearance
 1 Appearance black  
Genotype No Data  
Strain development 1998 by Koyasu at Keio Univ. School of Med.
Original :
Rag2tm1Fwa :1992 by F. W. Alt at Columbia Univ., N.Y. USA
IL2rbtm1Och:1995 by T. W. Mak at Ontario Cancer Institute, Toronto, Canada  
Strain description They lack T, B and NK cells. Furthermore, Macrophages and DCs have some functional defects.  
Colony maintenance Nothing special. Breed well.  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Il2rb  
Symbol name interleukin 2 receptor, beta chain  
Chromosome 15  
Common name CD122, IL-2 receptor beta chain, Il-2Rbeta, p70  
Symbol description No Data  
 2 Symbol Rag2  
Symbol name recombination activating gene 2  
Chromosome 2  
Common name Rag-2  
Symbol description No Data  
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)  
 3 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 15  
Common name neo; neomycin;  
Symbol description No Data  
 4 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 2  
Common name neo; neomycin;  
Symbol description No Data  
References Cell. 1992 Mar 6;68(5):855-67.
Eur J Immunol. 2001 Nov;31(11):3147-52.
J Immunol. 1996 Nov 15;157(10):4379-89.
Nat Immunol. 2001 Dec;2(12):1138-43.
Science. 1995 Jun 9;268(5216):1472-6.  
RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.
RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.
RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.
RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.
RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.
Research applications Cell Biology Research,
Immunology and Inflammation Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.  
Additional information
 1 Lab HP  
 2 Genotyping protocol <PCR>  
Depositor Koyasu, Shigeo (Keio University)  Koyasu, Shigeo 
Strain Status /
Availability
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