Strain Information | |
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Image | |
BRC No. | RBRC00672 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C.Cg-Stam<tm1Sug> Stam2<tm1Sug>/SugRbrc |
Former Common name | C.Cg-Stam<tm1Sug> Stam2<tm1Sug>, STAM1+/-, STAM2+/- |
H-2 Haplotype | |
ES Cell line | J1 [129S4/SvJae] |
Background strain | BALB/c |
Appearance | albino |
Strain development | Developed by Kazuo Sugamura, Tohoku University Graduate School of Medicine in 1999. The mutant mice were backcrossed to BALB/c over 6 times. |
Strain description | STAM1, STAM2 double knockout mice. STAM1 homozygous mutant mice show growth defect with aging, and neurological abnormality (reduction of pyramidal cells in CA3 of the hippocampus). STAM2 homozygous mutant mice are indistinguishable from controls. Double homozygous mutant mice are embryonic lethal. |
Colony maintenance | Stam: Heterozygote x Wild-type [or Crossing to BALB/cCrSlc]; Stam2: Homozygote x Homozygote [or Crossing to BALB/cCrSlc] |
References | Mol. Cell. Biol., 21, 3807-3819 (2001). 11340172 Mol. Cell. Biol., 22, 8648-8658 (2002). 12446783 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Stamsignal transducing adaptor molecule (SH3 domain and ITAM motif) 12Stam<tm1Sug>targeted mutation 1, Kazuo SugamuraSTAM1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Stam2signal transducing adaptor molecule (SH3 domain and ITAM motif) 22Stam2<tm1Sug>targeted mutation 1, Kazuo SugamuraSTAM2; 1200004O12Rik, 5730456G07Rik, Hbp Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP2loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP2loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)2neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | Phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse STAM1 and STAM2 genomic DNA |
Research application | Cre/loxP system Neurobiology Research |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall prior inform the DEPOSITOR of the purpose of the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Kazuo Sugamura (Miyagi Prefectural Hospital Organization) |
Strain Status | Frozen embryos |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |