Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: May 20, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
Figure
  Order
RBRC No. RBRC00694  
Type TransgeneCartagena   wks
Species Mus musculus  
Strain name B6.Cg-Tg(KRT14-Kitl)1Takk/TakkRbrc  
Former Common name hk14-SCF (human cytokeratin14 promoter + mouse stem cell factor cDNA) B6  
H-2 Haplotype No Data  
Background strain C57BL/6NCrSlc  
Appearance
 1 Appearance black and prominent epidermal pigmentation  
Genotype a/a B/B C/C Tg/+  
Strain development Developed by Dr. Takahiro Kunisada, Tottori University at 1996. The murine cDNA fragment under the human cytokeration 14 promoter was injected into the pronuclei of fertilized egges. The transgenic mice were maintained by brother-sister mating after backcrossing to C57BL/6Cr mice for 10 generations.
Generation:N10F20+F1F7  
Strain description These transgenic mice develop prominent epidermal pigmentation by overexpression of the mouse SCF gene controlled by the human keratin 14 promoter. The local high concentrations of soluble SCF have been implicated in the pathogenesis of human cutaneous mastocytosis. The phenotypes of these transgenic mice conform with that of human mastocytosis. This strain is more useful for studying of cutaneous mastocytosis and epidermal melanocytes biology than using of normal skin mice.  
Colony maintenance Sibling Mating (Hemizygote x Hemizygote, Wild-type)
Reproductive performance is good.  
Health Report No Data  
Gene Details
Promoter human cytokeratin14 promoter, poly A  
 1 Symbol Kitl  
Symbol name kit ligand  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References Development. 1998 Aug;125(15):2915-23.
J Exp Med. 1998 May 18;187(10):1565-73.  
Transgene expression of steel factor in the basal layer of epidermis promotes survival, proliferation, differentiation and migration of melanocyte precursors.
Transgene expression of steel factor in the basal layer of epidermis promotes survival, proliferation, differentiation and migration of melanocyte precursors.
Research applications No Data  
Specific Term and Conditions No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)  
Additional information
 1 Lab HP (Japanese)  
 2 Genotyping protocol <PCR>  
Depositor Kunisada, Takahiro (Gifu University, Graduate School of Medicine)  Kunisada, Takahiro 
Strain Status /
Availability
(Expected delivery)

back Back
RIKEN BioResource Research Center

English page click here ANIMAL SEARCH SYSTEM