Strain Data Sheet
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Data update: May 20, 2019
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RBRC No. RBRC00696  
Type TransgeneCartagena   10wks,♂
Species Mus musculus  
Strain name B6.Cg-Tg(KRT14-Edn3)1Takk/TakkRbrc  
Former Common name hk14-ET3 (human cytokeratin14 promoter + mouse endothelin 3 cDNA) B6  
H-2 Haplotype No Data  
Background strain C57BL/6NCrSlc  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C +/+  
 2 Appearance black with an aberrant pigmentation in the skin  
Genotype a/a B/B C/C Tg/?  
Strain development Developed by Dr. Takahiro Kunisada at Gifu University school of medicine in 1998. The mouse ET3 cDNA fragment under the human keratin 14 promoter was injected into the pronuclei of B6D2F1 fertilized eggs. The transgenic mice were maintained by brother-sister mating after backcrossing to C57BL/6Cr mice for 20 generations.  
Strain description These transgenic mice develop prominent epidermal pigmentation by overexpression of the mouse endothelin 3 gene controlled by the human keratin 14 promoter. The transgenic mice display a significant increase of the number of melanocytes, and increase thyroid tumorigenesis around 6 months old.  
Colony maintenance Sibling Mating (Hemizygote x Hemizygote, wild-type)
About 10% of Tg females has small body, and are hard to get pregnant.  
Health Report No Data  
Gene Details
Promoter human cytokeratin14 promoter  
 1 Symbol Edn3  
Symbol name endothelin 3  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References No Data  
Research applications No Data  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The DEPOSITOR should be a co-author in articles when the RECIPIENTs publish their data using these mice for 2 year after provided to the RIKEN BRC.  
Additional information
 1 Lab HP (Japanese)  
 2 Genotyping protocol <PCR>  
Depositor Kunisada, Takahiro (Gifu University, Graduate School of Medicine)  Kunisada, Takahiro 
Strain Status /
Availability
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