Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
Figure
  Order
RBRC No. RBRC00808  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.129P2-Emx1<tm1(cre)Ito>/ItoRbrc  
Former Common name Emx1-Cre  
H-2 Haplotype No Data  
ES cell line E14 [129P2/OlaHsd]  
Donor strain 129P2/OlaHsd via E14 ES cell line  
Background strain C57BL/6JJcl  
Appearance
 1 Appearance black (a/a B/B C/C)  
Genotype No Data  
Strain development Developed by Takuji Iwasato and Shigeyoshi Itohara, RIKEN Brain Science Institute. E14 ES cells were used to generate the mutant mice. The mice were backcrossed to C57BL/6J.  
Strain description Emx1-cre knock-in mice. neo type (inserted a cre recombinase gene and a frt flanked PGK-neo cassette into Emx1 gene)(RBRC00808), delta neo type (removed the pgk-neo cassette by FLP mediated recombination)(RBRC01345). The expression of cre recombinase was observed in dorsal telencephalon, neocortex, hippocampus, and olfactory bulb. In the delta neo type (RBRC01345), no ectopic cre expression was detected. Homozygous mice are viable and fertile.  
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Emx1  
Symbol name empty spiracles homolog 1 (Drosophila)  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 2 Symbol No Data  
Symbol name SV40 poly A  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 3 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 4 Symbol nls  
Symbol name SV40 large T antigen nuclear localization signal (NLS)  
Chromosome 6  
Common name No Data  
Symbol description No Data  
Promoter mouse Emx1 (Drosophila homeobox gene empty spiracles (Ems) mouse homolog) promoter  
 5 Symbol cre  
Symbol name Phage P1 Cre recombinase  
Chromosome 6  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 6 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 6  
Common name neo; neomycin;  
Symbol description No Data  
References Nature. 2000 Aug 17;406(6797):726-31.  
Cortex-restricted disruption of NMDAR1 impairs neuronal patterns in the barrel cortex.
Research applications Cell Biology Research,
Cre/loxP system,
FLP/frt system,
Neurobiology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nature, 406, 726-731 (2000).  
Additional information
 1 Lab HP  
 2 Mouse of the Month June 2006  
 3 Genotyping protocol <PCR>  
Depositor Itohara, ShigeYoshi (RIKEN Center for Brain Science)  Itohara, ShigeYoshi 
Strain Status /
Availability
(Expected delivery)

back Back
RIKEN BioResource Research Center

English page click here ANIMAL SEARCH SYSTEM