Strain Data Sheet
Data update: Aug 20, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
RBRC No. RBRC00869  
Type TransgeneCartagena   wks
Species Mus musculus  
Strain name B6.Cg-Tg(GOF18dPE/EGFP)48Ymat  
Former Common name B6.D2-Tg(GOF18 delta PE/EGFP)48, GOF18-delta PE-GFP line48 transgenic mouse  
H-2 Haplotype No Data  
Background strain C57BL/6JJcl  
 1 Appearance Black  
Genotype No Data  
Strain development Developed by Hans R. Shoeler University of Pennsylvania and Yasuhisa Mastui, Research Institute Oasaka Medical Center of Maternal and Child Health in 1997. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mutant mice were backcrossed to C57BL/6 over 3 times (RBRC00868 and RBRC01198). Backcrossed 129 for 7 time (RBRC01198) (by Atsuo Ogura, RIKEN BioResrouce center).  
Strain description Oct-4(Pou5f1)/EGFP transgenic mice carrying EGFP cDNA under the control of the 18kb Oct-4 genomic fragment (Genomic Oct-4 Fragment of 18) (Dev Growth Differ 1999). Oct-4 gene expresses in undifferenciated spermatogonia at prepubertal stages. This strain is useful to detect primordial germ cells with green fluorescence protein by the PGC-specific promoter. Tg line including the proximal enhancer (PE) of GOF18 (RBRC00868), Tg line lacking the proximal enhancer (PE) (RBRC00869 and RBRC01198).  
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl]  
Health Report No Data  
Gene Details
Promoter Oct 3/4 promoter  
 1 Symbol GFP  
Symbol name Green Fluorescent Protein (Jellyfish)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References Dev Growth Differ. 1999 Dec;41(6):675-84.  

Research applications Developmental Biology Research,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Dev. Growth. Differ., 41, 675-684 (1999).  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.  
 2 Genotyping protocol <PCR>  
Depositor Matsui, Yasuhisa  Matsui, Yasuhisa 
Strain Status /
(Expected delivery)

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