Strain Data Sheet
Data update: Aug 20, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
RBRC No. RBRC00886  
Type TransgeneCartagena   6wks,♂
Species Mus musculus  
Strain name B6;B6C3-Tg(Acro3-EGFP)01Osb  
Former Common name B6;C3 Tg(acro3-EGFP)01Osb (Nov. 2011), B6C3F1; B6C3F1 TgN(acro3-EGFP)/Osb01  
H-2 Haplotype No Data  
Background strain B6C3F1[C57BL/6N and C3H/HeN]  
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University. Acr3-EGFP transgene was injected into the pronuclei of B6C3F1 fertilized eggs. The mice were crossed to C57BL/6.  
Strain description Transgenic mice expressing GFP in the sperm acrosome from acr3-EGFP. Acr3-EGFP, in which EGFP with a proacrosin signal peptide and proacrosin N-terminal peptide were connected to the acrosin promoter.  
Colony maintenance Carrier x Carrier (Homozygote x Homozygote)  
Health Report
Gene Details
Promoter mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide  
 1 Symbol GFP  
Symbol name Green Fluorescent Protein (Jellyfish)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References FEBS Lett. 1999 Apr 23;449(2-3):277-83.  
Real-time observation of acrosomal dispersal from mouse sperm using GFP as a marker protein.
Research applications Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
FEBS Lett., 449, 277-283 (1999).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University ( The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP  
 2 Genotyping protocol <PCR>  
Depositor Okabe, Masaru (Osaka university)  Okabe, Masaru 
Strain Status /
(Expected delivery)

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