Strain Data Sheet
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Data update: Jun 20, 2019
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RBRC No. RBRC00891  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.129P2-Spen<tm1Hon>/HonRbrc  
Former Common name MINT-KO (C57BL/6 background)  
H-2 Haplotype No Data  
Donor strain 129P2/OldHsd via E14 ES cell line  
Background strain C57BL/6NCrSlc  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Dr. Tasuku Honjo, Kyoto University in 2003. A pgk-neo was transfered into E14 ES cells to replace nucleotide 4891 of the coding region of Mint. The Mint deficient mice were backcrossed to C57BL/6 for 16 times.  
Strain description B6.129P2-Mint-pending<tm1Hon>/HonRbrc. Mint, Msx2 interacting nuclear target protein which is a suppressor factor of a Notch/RBP-J signaling pathway play a role in fate determination in various lineages. Homozygous mutant mice are embryonic lethal due to developmental abnormality of heart, spleen and liver. This strain is useful for elucidating of Notch/RBP-J signaling pathway mechanism in mammals.  
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6CrSlc)
The reproductive performance is good. Homozygous mutant mice are embryonic lethal.  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Spen  
Symbol name Msx2-interacting nuclear target protein (MINT)  
Chromosome 4  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 2 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 4  
Common name neo; neomycin;  
Symbol description No Data  
References Immunity. 2003 Feb;18(2):301-12.  
Regulation of marginal zone B cell development by MINT, a suppressor of Notch/RBP-J signaling pathway.
Research applications Developmental Biology Research,
Immunology and Inflammation Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Immunity 18, 301-312 (2003).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.  
Additional information
 1 Honjo Lab HP  
 2 Genotyping protocol <PCR>  
Depositor Honjo, Tasuku (Graduate School of Medicine, Kyoto University)  Honjo, Tasuku 
Strain Status /
Availability
(Expected delivery)

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